ABSTRACT
AIM To develop a HPLC method for determining tectorigenin content in rabbit plasma for pharmacokinetics.METHODS As self crossover control,the rabbits,six Japanese giant ear rabbits were subject to a singledose intragastric administration of 60 mg/kg (tectorigenin 40 mg/kg) pueraria isoflavone.The free and enzymatic tectorigenin contents in plasma were measured by HPLC,and then pharmacokinetic parameters were calculated by PKsolver 2.0 pharmacokinetic program.RESULTS The AUC0-t,Tmax,Cmax after intragastric administration of pueraria isoflavone were (46.78 ±5.12) μg · min/mL,(5.39 ±0.54) min,(0.84 ±0.21) μg/mL for free tectorigenin,respectively;(485.48 ±23.53) μg · min/mL,(20.12 ±2.84) min,(2.95 ±0.67) μg/mL for total tectorigenin,respectively.CONCLUSION HPLC method is suitable for tectorigenin pharmacokinetic study.Tectorigenin is present mainly as glucuronide conjugates in plasma after intragastric administration pueraria isoflavone.
ABSTRACT
AIM To develop a HPLC method for determining tectorigenin content in rabbit plasma for pharmacokinetics.METHODS As self crossover control,the rabbits,six Japanese giant ear rabbits were subject to a singledose intragastric administration of 60 mg/kg (tectorigenin 40 mg/kg) pueraria isoflavone.The free and enzymatic tectorigenin contents in plasma were measured by HPLC,and then pharmacokinetic parameters were calculated by PKsolver 2.0 pharmacokinetic program.RESULTS The AUC0-t,Tmax,Cmax after intragastric administration of pueraria isoflavone were (46.78 ±5.12) μg · min/mL,(5.39 ±0.54) min,(0.84 ±0.21) μg/mL for free tectorigenin,respectively;(485.48 ±23.53) μg · min/mL,(20.12 ±2.84) min,(2.95 ±0.67) μg/mL for total tectorigenin,respectively.CONCLUSION HPLC method is suitable for tectorigenin pharmacokinetic study.Tectorigenin is present mainly as glucuronide conjugates in plasma after intragastric administration pueraria isoflavone.